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Literature summary extracted from

  • Dauk, M.; Lam, P.; Smith, M.
    The role of diacylglycerol acyltransferase-1 and phospholipid: diacylglycerol acyltransferase-1 and -2 in the incorporation of hydroxy fatty acids into triacylglycerol in Arabidopsis thaliana expressing a castor bean oleate 12-hydroxylase gene (2009), Botany, 87, 552-560.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.4 PCR-amplification, plant transformation by heat shock transformation of Agrobacterium tumefaciens (GV3101) with vector, expression in EMS mutant or T-DNA insertion lines of Arabidopsis thaliana deficient in the production of the enzymes diacylglycerol acyltransferase-1 (DGAT1), phospholipid: diacylglycerol acyltransferase-1 (PDAT1) and the homologue of PDAT1 phospholipid: diacylglycerol acyltransferase-2 (PDAT2) Ricinus communis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.4 additional information fusion enzyme of castor bean oleate 12-hydroxylase and tobacco cytochrome b5 Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.4 oleic acid esterified to membrane lipid phosphatidylcholine + reduced acceptor + H+ + O2 Ricinus communis
-
 hydroxy fatty acids in triacylglycerol + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.4 Ricinus communis D2CPE1 castor bean
-
2.3.1.20 Arabidopsis thaliana
-
-
-
2.3.1.158 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.4 seed
-
 Ricinus communis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.4 oleic acid esterified to membrane lipid phosphatidylcholine + reduced acceptor + H+ + O2
-
 Ricinus communis hydroxy fatty acids in triacylglycerol + acceptor + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.4 CFAH12 protein
-
 Ricinus communis
1.14.18.4 oleate 12-hydroxylase
-
 Ricinus communis
2.3.1.20 diacylglycerol acyltransferase-1
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.3.1.158 physiological function upon expression of heterologous oleate 12-hydroxylase in Arabidopsis thaliana mutants deficient in phospholipid:diacylglycerol acyltransferases 1 or 2 accumulate hydroxy fatty acids and show no difference with wild-type plants. Mutants are also able to accumulate hydroxy fatty acids in seed neutral lipids. Individually, phospholipid:diacylglycerol acyltransferases 1 or 2 do not play a major role in the incorporation of hydroxy fatty acids into triacylglycerols Arabidopsis thaliana